Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells.
作者： Amy Furda , Janine H. Santos , Joel N. Meyer , Bennett Van Houten
DOI: 10.1007/978-1-62703-739-6_31
关键词:
摘要: In this chapter, we describe a gene-specific quantitative PCR (QPCR)-based assay for the measurement of DNA damage, using amplification long targets. This has been used extensively to measure integrity both nuclear and mitochondrial genomes exposed different genotoxins proven be particularly valuable in identifying reactive oxygen species-mediated damage. QPCR can quantify formation damage as well kinetics removal. One main strengths is that it permits monitoring mtDNA directly from total cellular without need isolating mitochondria or separate step purification. Here discuss advantages limitations mammalian cells. addition, give detailed protocol helps facilitate its successful deployment any molecular biology laboratory.
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