Effects of aflatoxin B1 on DNA damage and P53geneamplification in hepatocyte-like cells differentiated from mesenchymal stem cells and CD34+ cellsobtained fromhuman umbilical cord blood
作者： Abdolamir Allameh , Masoud Soleimani , Masoumeh Ghaderi , Mehdi Fourozandeh-Moghadam , Shahnaz Ismaeili
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摘要: Background: Differentiation of mesenchymal stem cells (MSCs) to hepatocyte-li ke could be associated with development liver function factors. The impact differentiation-dependent changes on DNA integrity is not well understood. In this study, hepatocytes and their progenitor were treated aflatoxin B1 (AFB1) amplification selected genes linked damage was examined. Methods: MSCs CD34 + isolated from umbilical cord blood (UCB) AFB1 (0, 2.5, 10 20 µM) in selective media supporting the hepatocyte differentiation. After 24 htreatment (Comet assay) rates P53 �� -globin measured using real time polymerase chain reaction (QPCR). Results: results show that treatments resulted a concentration- dependent increase suppression specific gene amplification. extent significantly greater differentiated when compared those obtained cells. effects rate QPCR showed lesions (expressed as lesions/10 kb) derived Conclusions: These data together cytochrome P450 (CYP3A4) expression suggest non-differentiated are probably less vulnerable genotoxic agents them.
modares.ac.ir参考文章(26)
Amy Furda, Janine H. Santos, Joel N. Meyer, Bennett Van Houten, Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells. Methods of Molecular Biology. ,vol. 1105, pp. 419- 437 ,(2014) , 10.1007/978-1-62703-739-6_31
Olga A. Kovalenko, Janine H. Santos, Analysis of oxidative damage by gene-specific quantitative PCR. Current protocols in human genetics. ,vol. 62, ,(2009) , 10.1002/0471142905.HG1901S62
Marta Simonatto, Lucia Latella, Pier Lorenzo Puri, DNA damage and cellular differentiation: more questions than responses. Journal of Cellular Physiology. ,vol. 213, pp. 642- 648 ,(2007) , 10.1002/JCP.21275
I. L. Weissman, Translating Stem and Progenitor Cell Biology to the Clinic: Barriers and Opportunities Science. ,vol. 287, pp. 1442- 1446 ,(2000) , 10.1126/SCIENCE.287.5457.1442
Malcolm Alison, Catherine Sarraf, Hepatic stem cells Journal of Hepatology. ,vol. 29, pp. 676- 682 ,(1998) , 10.1016/S0168-8278(98)80165-7
Abdolamir Allameh, Somaieh Kazemnejad, Safety evaluation of stem cells used for clinical cell therapy in chronic liver diseases; with emphasize on biochemical markers. Clinical Biochemistry. ,vol. 45, pp. 385- 396 ,(2012) , 10.1016/J.CLINBIOCHEM.2012.01.017
Giel Hendriks, Fabienne Calléja, Harry Vrieling, Leon H.F. Mullenders, Jacob G. Jansen, Niels de Wind, Gene transcription increases DNA damage-induced mutagenesis in mammalian stem cells. DNA Repair. ,vol. 7, pp. 1330- 1339 ,(2008) , 10.1016/J.DNAREP.2008.04.015
Abdolamir Allameh, Shahnaz Esmaeli, Somaieh Kazemnejad, Masoud Soleimani, Differential expression of glutathione S-transferases P1-1 and A1-1 at protein and mRNA levels in hepatocytes derived from human bone marrow mesenchymal stem cells Toxicology in Vitro. ,vol. 23, pp. 674- 679 ,(2009) , 10.1016/J.TIV.2009.01.018
Sheng Yang, Ge Lin, Yue-Qiu Tan, Di Zhou, Lei-Yu Deng, De-Hua Cheng, Shu-Wei Luo, Tian-Cheng Liu, Xiao-Ying Zhou, Zheng Sun, Yang Xiang, Tian-Ji Chen, Ji-Fang Wen, Guang-Xiu Lu, Tumor progression of culture‐adapted human embryonic stem cells during long‐term culture Genes, Chromosomes and Cancer. ,vol. 47, pp. 665- 679 ,(2008) , 10.1002/GCC.20574
Anna Polesskaya, Michael A. Rudnicki, A MyoD-dependent differentiation checkpoint: ensuring genome integrity. Developmental Cell. ,vol. 3, pp. 757- 758 ,(2002) , 10.1016/S1534-5807(02)00372-6