Metabolic engineering of Ralstonia eutropha for the production of polyhydroxyalkanoates from sucrose.
作者： Si Jae Park , Young-Ah Jang , Won Noh , Young Hoon Oh , Hyuk Lee
DOI: 10.1002/BIT.25469
关键词:
摘要: A sucrose utilization pathway was established in Ralstonia eutropha NCIMB11599 and R. 437-540 by introducing the Mannheimia succiniciproducens MBEL55E sacC gene that encodes -fructofuranosidase. These engineered strains were examined for production of poly(3-hydroxybutyrate) [P(3HB)] poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)], respectively, from as a carbon source. It found -fructofuranosidase excreted into culture medium could hydrolyze to glucose fructose, which efficiently used sources recombinant strains. When expressing cultured nitrogen-free chemically defined containing 20g/L sucrose, high P(3HB) content 73.2wt% be obtained. In addition, Pseudomonas sp. MBEL 6-19 phaC1437 Clostridium propionicum pct540 accumulated P(3HB-co-21.5mol% LA) polymer 19.5wt% expression Escherichia coli ldhA gene. The molecular weights P(3HB-co-21.5mol%LA) synthesized using source 3.52x10(5) (M-n) 2.19x10(4) (M-n), respectively. reported here will useful polyhydroxyalkanoates (PHAs) one most abundant relatively inexpensive sources. Biotechnol. Bioeng. 2015;112: 638-643. (c) 2014 Wiley Periodicals, Inc.
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