作者: Diana Barthel , Birendra Singh , Kristian Riesbeck , Peter F Zipfel , None
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摘要: Pathogenic microbes acquire the human plasma protein plasminogen to their surface. In this article, we characterize binding of important coagulation regulator respiratory pathogen nontypeable Haemophilus influenzae and identify surface E (PE) as a new plasminogen-binding protein. Plasminogen binds dose dependently intact bacteria purified PE. The plasminogen–PE interaction is mediated by lysine residues also affected ionic strength. H. PE knockout strain (nontypeable 3655Δ pe ) bound with ∼65% lower intensity compared wild-type, PE-expressing strain. addition, expressed ectopically on Escherichia coli plasminogen. Plasminogen, either attached or PE, was accessible for urokinase activator. converted active plasmin cleaved synthetic substrate S-2251, natural substrates fibrinogen C3b. Using peptides that cover complete sequence protein, major region localized linear 28-aa-long N-terminal peptide, which represents aa 41–68. vitronectin, two proteins compete binding. Thus, Gram-negative bacterium , when plasmin, PE-bound aids in immune evasion contributes bacterial virulence.