Haemophilus influenzae Uses the Surface Protein E To Acquire Human Plasminogen and To Evade Innate Immunity

作者: Diana Barthel , Birendra Singh , Kristian Riesbeck , Peter F Zipfel , None

DOI: 10.4049/JIMMUNOL.1101927

关键词:

摘要: Pathogenic microbes acquire the human plasma protein plasminogen to their surface. In this article, we characterize binding of important coagulation regulator respiratory pathogen nontypeable Haemophilus influenzae and identify surface E (PE) as a new plasminogen-binding protein. Plasminogen binds dose dependently intact bacteria purified PE. The plasminogen–PE interaction is mediated by lysine residues also affected ionic strength. H. PE knockout strain (nontypeable 3655Δ pe ) bound with ∼65% lower intensity compared wild-type, PE-expressing strain. addition, expressed ectopically on Escherichia coli plasminogen. Plasminogen, either attached or PE, was accessible for urokinase activator. converted active plasmin cleaved synthetic substrate S-2251, natural substrates fibrinogen C3b. Using peptides that cover complete sequence protein, major region localized linear 28-aa-long N-terminal peptide, which represents aa 41–68. vitronectin, two proteins compete binding. Thus, Gram-negative bacterium , when plasmin, PE-bound aids in immune evasion contributes bacterial virulence.

参考文章(48)
Teresia Hallström, Anna M Blom, Peter F Zipfel, Kristian Riesbeck, None, Nontypeable Haemophilus influenzae Protein E Binds Vitronectin and Is Important for Serum Resistance Journal of Immunology. ,vol. 183, pp. 2593- 2601 ,(2009) , 10.4049/JIMMUNOL.0803226
Teresia Hallström, Elena Trajkovska, Arne Forsgren, Kristian Riesbeck, Haemophilus influenzae Surface Fibrils Contribute to Serum Resistance by Interacting with Vitronectin Journal of Immunology. ,vol. 177, pp. 430- 436 ,(2006) , 10.4049/JIMMUNOL.177.1.430
Elena Angelici, Carlo Contini, Massimiliano Spezzano, Roberto Romani, Paolo Carfagna, Pietro Serra, Rita Canipari, Plasminogen Activator Production in a Rat Model of Pneumocystis carinii Pneumonia Microbiology and Immunology. ,vol. 45, pp. 605- 611 ,(2001) , 10.1111/J.1348-0421.2001.TB01291.X
Sophia Poltermann, Anja Kunert, Monika von der Heide, Raimund Eck, Andrea Hartmann, Peter F Zipfel, None, Gpm1p Is a Factor H-, FHL-1-, and Plasminogen-binding Surface Protein ofCandida albicans Journal of Biological Chemistry. ,vol. 282, pp. 37537- 37544 ,(2007) , 10.1074/JBC.M707280200
L. Sottrup-Jensen, M. Zajdel, H. Claeys, T. E. Petersen, S. Magnusson, Amino-acid sequence of activation cleavage site in plasminogen: homology with "pro" part of prothrombin. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 72, pp. 2577- 2581 ,(1975) , 10.1073/PNAS.72.7.2577
C. P. Ponting, J. M. Marshall, S. A. Cederholm-Williams, Plasminogen: a structural review. Blood Coagulation & Fibrinolysis. ,vol. 3, pp. 605- 614 ,(1992) , 10.1097/00001721-199210000-00012
Paul Bresser, Ritva Virkola, Martina Jonsson-Vihanne, Henk M Jansen, Timo K Korhonen, Loek Alphen, Interaction of clinical isolates of nonencapsulated Haemophilus influenzae with mammalian extracellular matrix proteins. Fems Immunology and Medical Microbiology. ,vol. 28, pp. 129- 132 ,(2000) , 10.1111/J.1574-695X.2000.TB01466.X
Kaarina Lähteenmäki, Pentti Kuusela, Timo K. Korhonen, Bacterial plasminogen activators and receptors Fems Microbiology Reviews. ,vol. 25, pp. 531- 552 ,(2001) , 10.1111/J.1574-6976.2001.TB00590.X
Simone Bergmann, Sven Hammerschmidt, Fibrinolysis and host response in bacterial infections Thrombosis and Haemostasis. ,vol. 98, pp. 512- 520 ,(2007) , 10.1160/TH07-02-0117