Human pepsinogen C (progastricsin). Isolation of cDNA clones, localization to chromosome 6, and sequence homology with pepsinogen A.

摘要: The entire pepsinogen C (PGC) coding sequence was determined by analysis of a series five overlapping cDNA clones identified in library constructed from human gastric mucosa poly(A+) RNA. A partial clone initially using 256-fold degenerate oligonucleotide probe for amino acid residues 4-12 pepsin C, and subsequently 4 additional were upon rescreening with complementary to the 5' region original clone. Northern RNA PGC revealed an mRNA 1.5-kilobase species that indistinguishable detected (PGA) probe. In contrast, PGA probes distinct genomic restriction fragments indicating there no detectable cross-hybridization under high stringency conditions. gene localized chromosome 6 panel x mouse somatic cell hybrids. regions containing active site aspartyl groups are conserved relationship several other aspartic proteinases. We propose absence immunologic cross-reactivity between two pepsinogens, results divergent evolution sequences located on surface zymogens contrast strongly within binding cleft enzymes inaccessible antigenic recognition.