Requirements for zygotic gene activity during gastrulation in Drosophila melanogaster

摘要: Mutations at the folded gastrulation (fog) and twisted (tsg) loci interfere with early morphogenetic movements in Drosophila melanogaster. fog embryos do not form a normal posterior midgut although their germbands elongate, they extend dorsally. As result, when have fully extended germbands, mutant are into interior on ventral side of embryo. tsg abnormally deep dorsal folds during gastrulation, associated failure cells to slip laterally make way for expanding germband. Both continue develop, but disorganized first instar larvae. zygotically active genes expressed least by 10 20 min after onset gastrulation. mutations viable homozygous germ wild-type need be oogenesis survival heterozygous progeny. Elimination fog+ gene product from maternal does, however, affect extent folding observed heterozygotes. Analysis adult larval gynandromorphs indicates that function is only required region embryo, most probably giving rise or proctodeum. The relative mosaics suggests mosaic "lethal foci" also die embryogenesis, typical phenotype produced entire focus mutant. In contrast focus, no particular cell must wild type survival. Wild-type effective rescuing This may potentially which gives amnion serosa.