Rolling circle amplification of genomic templates for inverse PCR (RCA–GIP): a method for 5′- and 3′-genome walking without anchoring

作者: Athanasios Tsaftaris , Konstantinos Pasentzis , Anagnostis Argiriou

DOI: 10.1007/S10529-009-0128-9

关键词:

摘要: We have devised an improved method of genome walking, named rolling circle amplification of genomic templates for Inverse PCR (RCA–GIP). The method is based on the generation …

参考文章(14)
Steven D. Tanksley, Martin W. Ganal, Gregory B. Martin, Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes Trends in Genetics. ,vol. 11, pp. 63- 68 ,(1995) , 10.1016/S0168-9525(00)88999-4
Zefeng Yang, Xuefeng Wang, Shiliang Gu, Zhiqiu Hu, Hua Xu, Chenwu Xu, Comparative study of SBP-box gene family in Arabidopsis and rice. Gene. ,vol. 407, pp. 1- 11 ,(2008) , 10.1016/J.GENE.2007.02.034
Alexios N. Polidoros, Konstantinos Pasentsis, Athanasios S. Tsaftaris, Rolling circle amplification-RACE: a method for simultaneous isolation of 5' and 3' cDNA ends from amplified cDNA templates BioTechniques. ,vol. 41, pp. 35- 42 ,(2006) , 10.2144/000112205
Tokuji Tsuchiya, Nanako Kameya, Ikuo Nakamura, Straight walk: a modified method of ligation-mediated genome walking for plant species with large genomes. Analytical Biochemistry. ,vol. 388, pp. 158- 160 ,(2009) , 10.1016/J.AB.2009.02.002
Weining Tang, Sharyn E. Perry, Binding site selection for the plant MADS domain protein AGL15: an in vitro and in vivo study. Journal of Biological Chemistry. ,vol. 278, pp. 28154- 28159 ,(2003) , 10.1074/JBC.M212976200
Stefan de Folter, Gerco C. Angenent, trans meets cis in MADS science Trends in Plant Science. ,vol. 11, pp. 224- 231 ,(2006) , 10.1016/J.TPLANTS.2006.03.008
Athanasios S. Tsaftaris, Alexios N. Polidoros, Konstantinos Pasentsis, Apostolos Kalivas, Cloning, Structural Characterization, and Phylogenetic Analysis of Flower MADS-Box Genes from Crocus (Crocus sativus L.) The Scientific World Journal. ,vol. 7, pp. 1047- 1062 ,(2007) , 10.1100/TSW.2007.175
Lisa Stubbs, Long-range walking techniques in positional cloning strategies. Mammalian Genome. ,vol. 3, pp. 127- 142 ,(1992) , 10.1007/BF00352457
Apostolos Kalivas, Konstantinos Pasentsis, Alexios N. Polidoros, Athanasios S. Tsaftaris, Heterotopic expression of B-class floral homeotic genes PISTILLATA/GLOBOSA supports a modified model for crocus (Crocus sativus L.) flower formation. Dna Sequence. ,vol. 18, pp. 120- 130 ,(2007) , 10.1080/10425170601060582