Mapping of Epitopes Exposed on Intact Human Immunodeficiency Virus Type 1 (HIV-1) Virions: a New Strategy for Studying the Immunologic Relatedness of HIV-1

摘要: To study the antigenic conservation of epitopes human immunodeficiency virus type 1 (HIV-1) isolates different clades, abilities anti-HIV-1 gp120 and gp41 monoclonal antibodies (MAbs) to bind intact HIV-1 virions were determined by a newly developed virus-binding assay. Eighteen anti-HIV MAbs, which directed at V2, V3 loop, CD4-binding domain (CD4bd), C5, or regions, used. Nine from clades A, B, D, F, G, H Microtiter wells coated with after was added. Bound detected lysis testing for p24 antigen noncommercial enzyme-linked immunosorbent The anti-V3 MAbs strongly bound four clade B viruses non-B although binding weaker more sporadic latter. degrees CXCR4- CCR5-tropic similar, suggesting that loops these two categories are similarly exposed. anti-C5 D. Only weak all tested anti-CD4bd, anti-V2, anti-gp41 detected. These results suggest C5 structures shared well exposed on compared CD4bd, regions.