Optimal Transfection Conditions and the Safety Profiles of Dextran- Spermine/Plasmid DNA as a Potential Gene Transfer Vector to Mouse Airway
作者： A. J. Domb , W.Y. Wendy-Yeo , Syahrilnizam Abdullah , Rozita Rosli , Sabariah Abdul Rahman
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摘要: SUMMARY The emergence of gene therapy offers a new paradigm to the field molecular medicine. However, current viral and non-viral transfer vectors are not efficient often restricted by dose- limiting toxicity. Thus, generation delivery vector, which is with good safety profile highly required. In this study, optimal transfection conditions novel biodegradable cationic polymerdextran-spermine (D- SPM) in mouse airways were ascertained. highest level expression lungs BALB/c mice was detected at D-SPM plasmid DNA (pDNA) weight ratio (w/w) 16, 13.5 µg pDNA. No significant induction pro-inflammatory cytokines broncho alveolar lavage fluids observed, implies no overt toxicity occurred lungs. short, these results demonstrate that has moderate efficiency but acceptable airways.
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