Human p85 glycoprotein bears three distinct epitopes defined by several monoclonal antibodies
作者： Michelle Letarte , None
DOI: 10.1016/0161-5890(86)90101-X
关键词:
摘要: A glycoprotein of apparent mol. wt 85,000 isolated from human cells B lineage by affinity to 50B4-IgG immunoadsorbent was shown previously express two spatially distinct epitopes identified with monoclonal antibodies 50B4 and 50E6 [Letarte et al. (1985) Molec. Immun. 22, 113-124]. It is now demonstrated that the p85 homologous F10-44-2 antigen, defined initially as a T-lymphocyte-granulocyte-brain antigen [Dalchau (1980) Eur. J. 10, 745-749], A1G3 medullary thymocyte [Haynes (1983) 131, 1195-1200] A3D8 on erythrocytes [Telen clin. Invest. 71, 1878-1886]. The purified either lymphoblastoid cell line or leukemic line, at concn ranging 0.25 2.0 micrograms protein/ml, could block binding F-10-62-1 (a related antibody) cells. All these immunoprecipitated, antigenic preparation, single glycosylated polypeptide chain 85,000. Competitive studies indicated define least three epitopes. blocked reactivity 50E6-IgG thus bind an epitope identical epitope. Antibody F10-62-1 competed for recognizes 50B4-like antibody 30% but did not inhibit 50B4-IgG: it reacting third close
elsevier.com
sci-hub.se 参考文章(17)
M J Telen, R M Scearce, B F Haynes, E A Harden, M E Hemler, T J Palker, J L Strominger, G S Eisenbarth, Differentiation of human T lymphocytes. I. Acquisition of a novel human cell surface protein (p80) during normal intrathymic T cell maturation. Journal of Immunology. ,vol. 131, pp. 1195- 1200 ,(1983)
M Letarte, A Gougos, E J Quackenbush, R Baumal, Differential localization within human kidney of five membrane proteins expressed on acute lymphoblastic leukemia cells. Journal of Immunology. ,vol. 136, pp. 118- 124 ,(1986)
M Letarte, E J Quackenbush, Identification of several cell surface proteins of non-T, non-B acute lymphoblastic leukemia by using monoclonal antibodies. Journal of Immunology. ,vol. 134, pp. 1276- 1285 ,(1985)
Judy A. Falk, William H. Lewis, Michelle Letarte, Human Ia molecules carrying MT1 determinants: Purification with a monoclonal antibody Human Immunology. ,vol. 13, pp. 265- 280 ,(1985) , 10.1016/0198-8859(85)90005-9
Tony F. Cruz, Elizabeth J. Quackenbush, Michelle Letarte, Mario A. Moscarello, Effects of development and aging on the concentration of a human brain antigen Neuroscience Letters. ,vol. 59, pp. 253- 257 ,(1985) , 10.1016/0304-3940(85)90140-5
Peter N. Goodfellow, George Banting, Michael V. Wiles, Alan Tunnacliffe, Mohamed Parkar, Ellen Solomon, Rosemarie Dalchau, John W. Fabre, The gene, MIC4, which controls expression of the antigen defined by monoclonal antibody F10.44.2, is on human chromosome 11 European Journal of Immunology. ,vol. 12, pp. 659- 663 ,(1982) , 10.1002/EJI.1830120807
Tony F. Cruz, Elizabeth J. Quackenbush, Michelle Letarte, Mario A. Moscarello, Elevated levels of a glycoprotein antigen (P-80) in gray and white matter of brain from victims of multiple sclerosis. Neurochemical Research. ,vol. 11, pp. 877- 889 ,(1986) , 10.1007/BF00965211
Jun Okamura, Hiromitsu Okano, Yoshiko Ikuno, Hideko Tasaka, Seiji Motomura, Michelle Letarte, Erwin W. Gelfand, Characterization of new non-T, non-B acute lymphoblastic leukemia cell lines: Analysis of surface antigens by quantitative cellular radioimmunoassay and flow cytometry Leukemia Research. ,vol. 8, pp. 97- 104 ,(1984) , 10.1016/0145-2126(84)90037-7
Judith L. McKenzie, Rosemarie Dalchau, John W. Fabre, Biochemical characterisation and localization in brain of a human brain-leucocyte membrane glycoprotein recognised by a monoclonal antibody. Journal of Neurochemistry. ,vol. 39, pp. 1461- 1466 ,(1982) , 10.1111/J.1471-4159.1982.TB12592.X
U. Francke, B. E. Foellmer, B. F. Haynes, Chromosome mapping of human cell surface molecules: Monoclonal anti-human lymphocyte antibodies 4F2, A3D8, and A1G3 define antigens controlled by different regions of chromosome 11 Somatic Cell and Molecular Genetics. ,vol. 9, pp. 333- 344 ,(1983) , 10.1007/BF01539142