Chemical composition and tissue distribution of the human CDw44 glycoprotein.
作者： B F Flanagan , R Dalchau , J W Fabre , A K Allen , A S Daar
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摘要: The CDw44 glycoprotein was purified from 2.3 x 10(11) CD3+ CD4+ CD8- T-chronic lymphocytic leukaemia (CLL) cells using F10-44-2 monoclonal antibody affinity chromatography, DEAE-Sepharose anion-exchange passage down carboxymethyl (CM)-Sepharose cation-exchange columns, wheat germ lectin chromatography and gel-permeation chromatography. On elution in non-ionic detergents the DEAE column, two distinct peaks of antigen activity were obtained. each peak a 85,000 MW, but amino acid composition noticeably different. Carbohydrate compositions showed that contained approximately 30% (w/w) carbohydrate, suggesting both O-linked complex N-linked glycans. Modulation studies with on normal peripheral blood mononuclear (PBMC) demonstrated T consisted one fraction readily modulated, other which resistant to modulation. Detailed tissue distribution for performed frozen sections human tissues. has restricted distribution, is found many highly diverse cell types (e.g. lymphocytes, smooth muscle cells, some secretory glands, skin epithelial cells).
参考文章(5)
A K Allen, N N Desai, A Neuberger, Purification of the glycoprotein lectin from the broad bean (Vicia faba) and a comparison of its properties with lectins of similar specificity Biochemical Journal. ,vol. 155, pp. 127- 135 ,(1976) , 10.1042/BJ1550127
A.S. Daar, John W. Fabre, The membrane antigens of human colorectal cancer cells: Demonstration with monoclonal antibodies of heterogeneity within and between tumours and of anomalous expression of HLA-DR European Journal of Cancer and Clinical Oncology. ,vol. 19, pp. 209- 220 ,(1983) , 10.1016/0277-5379(83)90419-4
Bj⊘rn O. Eggum, Determination of Tryptophan Acta Agriculturae Scandinavica. ,vol. 18, pp. 127- 131 ,(1968) , 10.1080/00015126809433166
CD Breder, CA Dinarello, CB Saper, Interleukin-1 immunoreactive innervation of the human hypothalamus Science. ,vol. 240, pp. 321- 324 ,(1988) , 10.1126/SCIENCE.3258444
Michelle Letarte, None, Human p85 glycoprotein bears three distinct epitopes defined by several monoclonal antibodies Molecular Immunology. ,vol. 23, pp. 639- 644 ,(1986) , 10.1016/0161-5890(86)90101-X