Hyaluronate-CD44 Interactions Can Induce Murine B-Cell Activation

摘要: CD44 is a widely distributed cell surface glycoprotein whose principal ligand has been identified as hyaluronic acid (HA), major component of the extracellular matrix (ECM). Recent studies have demonstrated that activation through leads to induction effector function in T cells and macrophages. In current study, we investigated whether HA or monoclonal antibodies (MoAbs) against would induce proliferative response mouse lymphocytes. Spleen from normal nude, but not severe combined immunodeficient mice, exhibited strong responsiveness stimulation with soluble anti-CD44 MoAbs. Furthermore, purified B cells, were found respond HA. was unable stimulate even presence antigen presenting (APC) act costimulus mitogenic submitogenic concentrations anti-CD3 contrast, vitro, led B-cell differentiation measured by production IgM addition increased expression decreased levels CD45R. The fact responding directly its binding any contaminants endotoxins F(ab)2 fragments MoAbs fusion proteins could significantly inhibit HA-induced proliferation cells. Also, affected polymixin B, lipopolysaccharide (LPS)-unresponsive C3H/HeJ strain responded strongly HA, chondroitin-sulfate, another ECM, induced activation. It also noted injection intraperitoneally, triggered splenic vivo. Together, study demonstrates interaction between can regulate murine functions such interactions may play critical role during autoimmune