Rapid Morphological and Cytoskeletal Response to Microgravity in Human Primary Macrophages

摘要: The FLUMIAS (Fluorescence-Microscopic Analyses System for Life-Cell-Imaging in Space) confocal laser spinning disk fluorescence microscope represents a new imaging capability live cell experiments on suborbital ballistic rocket missions. During the second pioneer mission of this system TEXUS-54 flight, we developed and performed experiment with primary human macrophages. We simultaneously imaged four different cellular structures (nucleus, cytoplasm, lysosomes, actin cytoskeleton) by using dyes (Nuclear Violet, Calcein, LysoBrite, SiR-actin) wavelengths (405, 488, 561, 642 nm), investigated morphology microgravity (10−4 to 10−5 g) over period about six minutes compared 1 g controls. For cytoskeleton during spaceflight, combined microscopy SiR-actin probe, fluorogenic silicon-rhodamine (SiR) conjugated jasplakinolide probe that binds F-actin displays minimal toxicity. determined changes 3D volume surface, nuclear cytoskeleton, which responded rapidly environment significant reduction after 4–19 s microgravity, adapted subsequently until 126–151 microgravity. conclude induces geometric rapid response adaptation potential gravity-transducing