Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPARγ

摘要: Accumulating data have shown that the microenvironment of dendritic cells modulates subtype differentiation and CD1 expression, but mechanisms by which exogenous factors confer these effects are poorly understood. Here we describe dependence CD1a- monocyte-derived cell (moDC) development on lipids associated with expression peroxisome proliferator-activated receptor-gamma (PPARgamma). We also show consecutive immature CD1a-PPARgamma+ moDCs to CD1a+PPARgamma- limited serum lipoproteins terminated proinflammatory cytokines. Immature possess higher internalizing capacity than CD1a+ cells, whereas both activated subtypes similar migratory potential differ in their cytokine chemokine profiles, translates distinct T-lymphocyte-polarizing capacities. stand out capability secrete high amounts IL-12p70 CCL1. As skew moDC toward generation inhibit suggest uptake results endogenous PPARgamma agonists induce a cascade gene transcription coordinating lipid metabolism, lipid-presenting molecules, dichotomy, function. The presence DCs lymph nodes pulmonary Langerhans histiocytosis confirms functional relevance DC subsets vivo.