Dual nuclease activity of a Cas2 protein in CRISPR-Cas subtype I-B of Leptospira interrogans.
作者： Bhuvan Dixit , Karukriti Kaushik Ghosh , Gary Fernandes , Pankaj Kumar , Prerana Gogoi
关键词:
摘要: Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 carries a set of cas genes associated with CRISPR-Cas subtype I-B. Herein, we report for the first time active transcription (cas1 to cas8) L. where cas4, cas1, cas2 and cas6, cas3, cas8, cas7, cas5 are clustered together in two independent operons. As an initial step toward comprehensive understanding system spirochete, biochemical study one core Cas2 proteins (Lep_Cas2) showed nuclease activity on both DNA RNA nonspecific manner. Additionally, unlike other known proteins, Lep_Cas2 metal-independent RNase preferential over DNA. These results provide insight diversity existing prokaryotic adaptive immune system.
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