作者: Johannes Willem Hofstraat
DOI:
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摘要: The invention relates to a method for detection of an analyte in test sample by specific binding reaction among the analyte, partner and (immuno)reactant provided with label, characterized that label is lanthanide ion-ligand complex wherein ion neodymium(III) (Nd3+), ytterbium(III) (Yb3+), or erbium(III) (Er3+) ligand comprises contact sensitizing moiety which absorbs 400-1000 nm region, preferably 400-800 region. Further, diagnostic kit disclosed as well detecting matrix biomedical interest through oligonucleotide, antigen, antibody attached material, core-shell latex sites antigen labeled brought into after lanthanide-ion immobilized on and, optionally, residual removed, obtained irradiated light emitted from said detected if present interest.