Relative ability of modified versions of the hamster oocyte penetration test, incorporating hyperosmotic medium or the ionophore A23187, to predict IVF outcome.

摘要: This study was designed to assess the relationship between IVF outcome and results obtained with two modified versions of zona-free hamster oocyte penetration test in which spermatozoa were pre-incubated either hyperosmotic medium or divalent cation ionophore A23187. When former system used, a poor correlation observed. Samples screened prior exhibited 60% false negative rate (failed test, successful IVF), while for those assessed concurrently IVF, equivalent figure 85.7%. Addition A23187 optimized giving higher levels sperm--oocyte fusion more accurate prediction capacity fertilize human ova vitro. With this 4.3% samples 0% simultaneously IVF. These suggest that A23187-enhanced may be value as screening criterion