Colony-forming assay for circulating chronic lymphocytic leukemia cells

摘要: In these studies, we report adaptation of a colony-forming assay to chronic lymphocytic leukemia (CLL) peripheral blood cells. T-lymphocyte-depleted CLL cells were cultured with irradiated, normal T and media conditioned by normal, mitogen-stimulated in methylcellulose. Colonies containing small transformed lymphocytes appeared after 5-7 days incubation. The plating efficiency colonies was 0.15 +/- 0.08% (x S.D.), similar that other systems. majority S phase (50 4% x S.E.) as determined thymidine suicide the fraction inversely related WBC. Cells harvested from lacked surface markers for stained positively monoclonal and/or cytoplasmic immunoglobulin light chains. A 1-h incubation used study vitro response adriamycin melphalan. Preliminary studies suggest differences patterns sensitivity melphalan between patients previously treated alkylating agents those who had not received treatment. This system can be regulation cell proliferation, may have utility predicting chemotherapeutic agents.