Sensitive silver staining of proteins after isoelectric focusing in agarose gels
作者： Olof Vesterberg , Birte Gramstrup-Christensen
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摘要: As isoelectric focusing in agarose gels has many merits it is valuable to be able visualize proteins at concentrations lower than detectable with Coomassie Brilliant Blue R-250 staining. A silver staining procedure been adapted such and including serum samples have studied. Silver gave very sharp protein bands was about 30 times more sensitive This important allow direct application of dilute solutions as urine cerebrospinal fluid. may also avoid artifacts e.g. caused by concentration methods.
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