Surface adhesion and attachment factors in bone morphogenetic protein-induced chondrogenesis in vitro.

摘要: Bone morphogenetic protein (BMP) induces cartilage and bone development in embryonic post-fetal life. protein-induced chondrogenesis outgrowths of muscle connective tissues on various furrowed unfurrowed fibronectin-coated substrata was observed by biochemical histologic methods. The consisted cellulose acetate membranes, atelocollagen, denatured autoclaved demineralized matrix, matrix deactivated extraction BMP with guanidine hydrochloride (GuHCl), aggregates insoluble associated noncollagenous proteins (BMP/NCP) prepared from bovine bone. furrows, which were mechanically cut into the substrata, intended to increase surface area provide extra spaces for cell proliferation under compression. extent extracellular attachments required induced reflected quantity formed when BMP/NCP, either or water-soluble form, introduced greatest GuHCl-extracted matrix. On BMP-induced relatively scanty. A substratum autoclaving at a minimum 125 degrees, permitted cell-to-cell adhesion but not cell-to-substratum attachments; end product loose fibrous tissue only. In contrast, occurred surfaces undissolved particles BMP/NCP. Water-soluble human masses amorphous cartilage. Even after it extracted GuHCl, rat may have retained trace amounts endogenous BMP. Thus, requirements cells attachment, including mechanical factors such as furrows enlarge area, met, manifestation temporal, spatial, distribution patterns. situ hybridization immunofluorescent microscopy aid antirecombinant antibiotics new information about morphogenesis.