Tissue-engineered cartilage using serially passaged articular chondrocytes. Chondrocytes in alginate, combined in vivo with a synthetic (E210) or biologic biodegradable carrier (DBM).

摘要: In vitro multiplication of isolated autologous chondrocytes is required to obtain an adequate number cells generate neo-cartilage, but known induce cell-dedifferentiation. The aim this study was investigate whether multiplied can be used neo-cartilage in vivo. Adult bovine articular chondrocytes, various differentiation stages, were suspended alginate at densities 10 or 50 million/ml, either directly after isolation (P0) monolayer for one (P1) three passages (P3). Alginate with seeded demineralized bone matrix (DBM) a fleece polylactic/polyglycolic acid (E210) and implanted nude mice 8 weeks. newly formed tissue evaluated by Alcian Blue immunohistochemical staining collagen type-II type-I. Structural homogeneity the tissue, composed freshly as well serially passaged cells, found enhanced high-density seeding (50 million/ml) use E210 carrier. percentage positive P3-cells generally higher when Furthermore, P3-chondrocytes highest density expression. This shows promising possibilities structurally regular using combination acid.