Differentiation in cultured limbal epithelium as defined by keratin expression.

摘要: The authors investigated differentiation of cultured corneal and limbal epithelial cells by immunochemically evaluating the changes in profiles keratins recognized two monoclonal antibodies: AE5, which recognizes K3, AE1, a group acidic including K16, is present hyperproliferative cells. After 1 2 weeks culture, human did not react with AE5 but strongly AE1. At 3 weeks, only suprabasal exhibited moderate reactivity whereas AE1 binding was seen all 5 to 6 reacted moderately Treatment 2-week-old limbally derived cultures mitomycin C (mitosis inhibitor) inhibit subsequent K3 expression. Thus, expression associated maturation or later stage that require an additional cell division. Unlike cells, rabbit expressed (reactivity AE5) after ten days culture. epithelium from central cornea lost week tissue culture keratin(s) Even 4-6 become confluent AE5. undergo chronological similar cornea. However, take significantly longer time express (a phenotypic characteristic differentiated epithelium) than do