Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells.

摘要: Objectives It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence pathophysiology of several ocular disorders, such as neovascularization. This study assessed impact ELF-PEMF on proangiogenic features RPE cells. Materials methods Primary cultured were treated with (50 Hz) for three days. Using ELISA assay, we evaluated effects treatment cell proliferation apoptosis. Also, RT-PCR was used to determine gene expression factors, matrix metalloproteinase-2 (MMP-2), MMP-9, vascular endothelial growth factors receptor 2 (VEGFR-2), hypoxia-inducible factor 1 (HIF-1α), VEGFA, cathepsin D, connective tissue (CTGF), E2F3, inhibitors metalloproteinases (TIMP-1), TIMP-2. Results No noticeable changes observed death ELF-PEMF-exposed cells, while transcript levels genes (HIF-1α, VEGFR-2, CTGF, TIMP-1, MMP-2, MMP-9) increased significantly. Conclusion are important homeostasis retina. which highlights concerns about this human health.