Silver Staining of Senile Plaques and Neurofibrillary Tangles in Paraffin Sections: A Simple and Effective Method

摘要: Summary A one-step silver staining method using a protective colloidal developer of gelatin and formic acid was originally established for demonstration argyrophilic nucleolus organizer regions by controlled reduction silver. We describe here new application this technique that can easily be performed to demonstrate senile plaques (SP) neurofibrillary tangles (NFT) on paraffin sections. Preliminary results in ten cases dementia Alzheimer type five amyloid congophilic angiopathy showed reliable neuritic SP as well NFT all 15 cases. In addition, deposits perivascular areas fibrillar amyloid, the diffuse plaques, were revealed. The success seems depend particularly low concentration developer, which might responsible agent careful revealing buried structures NFT. features quite similar those with anti-β anti-tau immunostaining. This result suggests high specificity extracellular intraneuronal cerebral amyloid. detailed delicate morphological points sensitivity amyloids, tangles, respectively, simple inexpensive method.