Opsonin-Independent Phagocytosis of Activators of the Alternative Complement Pathway by Human Monocytes

摘要: Activators of the alternative C pathway were ingested by human monocytes in a synthetic medium devoid serum or proteins pathway. The rank order percentage ingesting rabbit, mouse C57B, and CBA erythrocytes was same as that observed for lysis these C2-deficient diluted GVB-Mg-EGTA. Sheep guinea pig erythrocytes, which not monocytes, lysed Although capacity to ingest rabbit consistent particular donor, there bimodal distribution among donors terms involved. Zymosan ingestion, on other hand, with high proportion from all donors. zymosan particles largely diminished when pretreated quantities affinity-purified trypsin, had little no effect their ability phagocytose EA even bind E(IgM)C43b intermediates. Latex did activate after treatment concentration trypsin 10 times required reduce substantially ingestion zymosan. trypsin-sensitive mechanism involved regenerated over 48 hr during vitro culture is result compatible restoration function membrane protein. particulate activators absence surface-associated opsonizing such IgG C3b step represents monocyte recognition specific activating surfaces