The chlorinating potential of the human monocyte.

摘要: Human monocytes incubated with phorbol myristate acetate (PMA) or opsonized zymosan particles can chlorinate the beta-amino acid taurine to its monochloramine derivative. Taurine then be quantitated by ability oxidize 5-thio-2-nitrobenzoic disulfide characteristic absorption peak at 252 nm. Stimulated, but not resting, chlorinated a process dependent on time, cell concentration, and pH. The formation of chloramine stimulated could inhibited catalase, azide, cyanide, was unaffected superoxide dismutase, exogenous myeloperoxidase. Thus, generation human appeared both H2O2 Compared neutrophils, monocyte generate similar amounts when acetate, far less if were used as trigger. Based known H2O2- myeloperoxidase-Cl- system free HOCl, it would seem that this oxidant is most likely species responsible for monocyte- mediated chlorination reactions. we have simple quantitative assay demonstrate large quantities highly reactive toxic oxygen metabolite.