Fermentation of methanethiol and dimethylsulfide by a newly isolated methanogenic bacterium

摘要: From dilution series in defined mineral medium, a marine iregular coccoid methanogenic bacterium (strain MTP4) was isolated that able to grow on methanethiol as sole source of energy. The strain also grew dimethylsulfide, mono-, di-, and trimethylamine, methanol acetate. On formate the organism produced methane without significant growth. Optimal growth MT, with doubling times about 20 h, occurred at 30°C medium. isolate required p-aminobenzoate further not identified vitamin. Strain MTP4 had high tolerance hydrogen sulfide but very sensitive mechanical forces or addition detergents such Triton X-100 sodium dodecylsulfate. Methanethiol fermented by according following equation: 1 $$\begin{array}{*{20}c} {4CH_3 SH + 3H_2 O \to 3CH_4 HCO_3^ - } \\ { 4HS^ 5H^ {(\Delta G^{{\rm O}{\rm I}} = 36.9kJ/MT)} \end{array} $$ yield 3.06 g cell dry mass per mol MT. During MT released small amounts DMS and, vice versa, degradation accompanied intermediate production.