Effect of medroxyprogesterone acetate and norethisterone on serum-stimulated and estradiol-inhibited proliferation of human coronary artery smooth muscle cells.

摘要: OBJECTIVE: The addition of progestogen to estrogen replacement therapy is thought antagonize, at least in part, the beneficial effects estrogens on vasculature. aim this study was investigate effect two progestogens mostly used clinical practice proliferation vascular smooth muscle cells, which has been demonstrated be a crucial step development atherosclerosis. MATERIAL AND METHODS: medroxyprogesterone acetate (MPA) and norethisterone (NET), represent different classes C21- C19-progestogens, respectively, investigated cells from human coronary artery vitro. steroids were tested concentration range 10(-8) 10(-5) M, upper that reached during hormonal therapy, compared with control values. RESULTS: Estradiol significantly inhibited serum-stimulated cell growth concentrations 10(-6) M by 18% 34%, respectively. MPA enhanced 29% 47%, In equimolar combinations estradiol, stimulated 26% 44%, contrast, NET had no significant impact estradiol-inhibited proliferation. CONCLUSIONS: These data suggest may antagonize antiatherosclerotic estradiol vasculature, whereas neutral respect. However, these occurred supraphysiological concentrations. Because might target tissues, relevance for treatment patients cardiovascular risk cannot excluded.