A method of purification of partially methylated alditol acetates in the methylation analysis of glycoproteins and glycopeptides
作者： Mark E. Lowe , Bo Nilsson
DOI: 10.1016/0003-2697(84)90323-3
关键词:
摘要: Abstract A method for methylation analysis of intact glycoproteins is described. Starting with glycoprotein, the oligosaccharides are methylated, hydrolyzed, reduced, and acetylated. The partially methylated alditol acetates then separated from noncarbohydrate contaminants on a silica gel G column. Partially hexitol eluted column petroleum ether:ethyl acetate (1:1, v ) N-acetylhexosaminitol subsequently methanol. Analysis by gas-liquid chromatography/mass spectrometry shows no interfering contaminants. This circumvents need to make pronase glycopeptides avoids pitfalls other procedures.
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