Bioreactor Engineering For Recombinant Protein Production Using Plant Cell Suspension Culture

摘要: Plant cell culture has long been considered as a potential system for large-scale production of secondary metabolites. In recent years, with the advances in plant molecular biology, also attracted considerable interests an expression platform high-value recombinant proteins. Many species can now be genetically transformed. Callus cells derived from transgenic plants grown simple, chemically defined liquid media to establish suspension cultures protein production. For certain species, such tobacco, it is possible by directly transforming wild-type cultured cells. There are several notable benefits using cells, unlike prokaryotic hosts, capable performing complex post-translational processing, propeptide signal peptide cleavage, folding, disulfide bond formation and glycosylation, which required active biological functions expressed heterologous proteins [1]. easier less expensive cultivate than their mammalian or insect counterparts. The human pathogen contamination problem associated does not exist since used [2]. When compared plants, possess number advantages. Cultured have much shorter growth cycle that field. confined environment (i.e. enclosed bioreactor) hence devoid GMO release problem. Furthermore, consist dedifferentiated callus lacking fully functional plasmodesmata there minimum cell-to-cell communication. This may reduce systemic post-transcriptional gene silencing (PTGS) believed transmitted via vascular [3,4]. On down side, generally longer doubling time bacterial yeast Genetic instability de-differentiated due somaclonal variation another drawback Due part more evolved tightly controlled gene/protein