Identification of underexpressed genes in early- and late-stage primary ovarian tumors by suppression subtraction hybridization

摘要: To identify novel tumor suppressor genes involved in ovarian carcinogenesis, we generated four down-regulated suppression subtraction cDNA libraries from two early-stage (stage I/II) and late-stage III) primary tumors, each subtracted against cDNAs derived normal epithelial cell brushings. Approximately 600–700 distinct clones were sequenced library. Comparison of obtained early- tumors revealed that unique to library which suggested tumor-specific differences. We found 45 common all libraries. also identified several genes, the role development has yet be elucidated, addition under expressed potential carcinogenesis been described previously (Bagnoli et al ., Oncogene, 19: 4754–4763, 2000; Yu Proc. Natl. Acad. Sci. USA, 96: 214–219, 1999; Mok 12: 1895–1901, 1996). The differential expression a subset these was confirmed by semiquantitative reverse transcription-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control panel 15 stage I III mixed histological subtypes. Chromosomal sorting sequences mapped known regions deletion cancer. Loss heterozygosity (LOH) analysis multiple genomic with high frequency loss both tumors. determine whether some corresponds an allele LOH, used microsatellite marker for one on 8q have shown this gene correlates LOH. In conclusion, our map well deletions may represent candidate