Generation of antigen-specific Th2 cells from unprimed mice in vitro: effects of dexamethasone and anti-IL-10 antibody.

摘要: We describe a system for the in vitro production of Ag-specific mouse CD4 cell lines from unprimed mice. Purified + CD45RB high T cells were exposed to Ag-pulsed accessory serum-free medium 24 h; cultured absence Ag and presence serum, IL-2, dexamethasone, Abs IL-10 an additional 4 days; then re-exposed original sensitizing Ag. The dexamethasone during initial expansion stage appeared be critical ability stimulated expanded respond restimulation with same Repeated cycles stimulation led increased specificity (in current case, pigeon cytochrome c ), decline IL-2 IFN-γ, IL-4, IL-5, IL-10. This culture protocol provides test exploration factors that regulate conversion naive memory development specific immune responses protein Ags. data are consistent models implicate glucocorticoids as regulators response specificity.