MATURATION OF RECEPTOR PROTEINS IN EUKARYOTIC EXPRESSION SYSTEMS
作者： Hamid M. Sadeghi , Giulio Innamorati , Mariel Birnbaumer
DOI: 10.3109/10799899709036619
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摘要: AbstractTransient and stable expression in eukaryotic cells is commonly used to examine receptor function. Characterization of the V2 vasopressin synthesized transiently transfected revealed presence large quantities immature protein a small fraction fully mature protein. The was characterized by its sensitivity endoglycosidase H treatment, abnormal migration SDS PAGE, tendency form aggregates. Prevention glycosylation mutagenesis increased produced, but did not eliminate need for maturation step. On other hand, stably produce almost exclusively with t1/2 6 h, while has 20 min. In absence N-linked reduced 4.5 h. transient experiments proteins exhibited prolonged about 8 Co...
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