Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience.
作者： F. Malentacchi , C.M. Ciniselli , M. Pazzagli , P. Verderio , L. Barraud
DOI: 10.1016/J.CCA.2014.12.004
关键词:
摘要: Abstract Background DNA integrity is a critical part of the definition genomic (gDNA) quality and can influence downstream molecular applications. Pre-analytical variables as sample storage extraction methods quantity isolated affect test performances. The aim this paper to investigate role blood procedures on gDNA fragmentation impact test. Methods 157 samples deriving from Pan European 1st SPIDIA External Quality Assessment (EQA), aimed quantity, have been analyzed by Pulsed Field Gel Electrophoresis ImageJ imaging software. derived which Results/conclusions Our results demonstrate that same underwent long range multiplex PCR based analytical provide different if adopted pre-analytical are not standardized.
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sci-hub.se 参考文章(26)
Agnelo Furtado, DNA extraction from vegetative tissue for next-generation sequencing Methods of Molecular Biology. ,vol. 1099, pp. 1- 5 ,(2014) , 10.1007/978-1-62703-715-0_1
R. Artusi, P. Verderio, E. Marubini, Bravais-Pearson and Spearman correlation coefficients: meaning, test of hypothesis and confidence interval International Journal of Biological Markers. ,vol. 17, pp. 148- 151 ,(2002) , 10.5301/JBM.2008.2127
Thomas Knebelsberger, Isabella Stöger, DNA Extraction, Preservation, and Amplification Methods of Molecular Biology. ,vol. 858, pp. 311- 338 ,(2012) , 10.1007/978-1-61779-591-6_14
Debra McLaggan, Noppadon Adjimatera, Kristina Sepčić, Marcel Jaspars, DavidJ MacEwan, IanS Blagbrough, RoderickH Scott, Pore forming polyalkylpyridinium salts from marine sponges versus synthetic lipofection systems: distinct tools for intracellular delivery of cDNA and siRNA. BMC Biotechnology. ,vol. 6, pp. 6- 6 ,(2006) , 10.1186/1472-6750-6-6
David Ravine, Graeme Suthers, Quality standards and samples in genetic testing. Journal of Clinical Pathology. ,vol. 65, pp. 389- 393 ,(2012) , 10.1136/JCLINPATH-2011-200519
F. Malentacchi, M. Pazzagli, L. Simi, C. Orlando, R. Wyrich, C.C. Hartmann, P. Verderio, S. Pizzamiglio, C.M. Ciniselli, A. Tichopad, M. Kubista, S. Gelmini, SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses Clinica Chimica Acta. ,vol. 424, pp. 274- 286 ,(2013) , 10.1016/J.CCA.2013.05.012
Nicola A Johnson, Rifat A Hamoudi, Koichi Ichimura, Lu Liu, Danita M Pearson, V Peter Collins, Ming-Qing Du, Application of array CGH on archival formalin-fixed paraffin-embedded tissues including small numbers of microdissected cells Laboratory Investigation. ,vol. 86, pp. 968- 978 ,(2006) , 10.1038/LABINVEST.3700441
Mark M. Davis, Pamela J. Bjorkman, T-cell antigen receptor genes and T-cell recognition. Nature. ,vol. 334, pp. 395- 402 ,(1988) , 10.1038/334395A0
Aylin Üstündağ, Kemal Şimşek, Hakan Ay, Kadir Dündar, Sinan Süzen, Ahmet Aydın, Yalçın Duydu, DNA integrity in patients undergoing hyperbaric oxygen (HBO) therapy Toxicology in Vitro. ,vol. 26, pp. 1209- 1215 ,(2012) , 10.1016/J.TIV.2012.06.007
Maryam Shabihkhani, Gregory M. Lucey, Bowen Wei, Sergey Mareninov, Jerry J. Lou, Harry V. Vinters, Elyse J. Singer, Timothy F. Cloughesy, William H. Yong, The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings. Clinical Biochemistry. ,vol. 47, pp. 258- 266 ,(2014) , 10.1016/J.CLINBIOCHEM.2014.01.002