Validation of an Engineered Cell Model for In Vitro and In Vivo HIF-1α Evaluation by Different Imaging Modalities
作者： A. Lo Dico , S. Valtorta , C. Martelli , S. Belloli , U. Gianelli
DOI: 10.1007/S11307-013-0669-0
关键词:
摘要: The aim of this study was to characterize a cell-based model for the molecular hypoxia-inducible factor (HIF)-1α activity, in context hypoxia, by means different imaging techniques. Engineered U251-HRE glioma cells were used analyze mechanisms underlying HIF-1α activity vitro relation luciferase expression. same orthotopically implanted mice evaluate tumor progression and hypoxia induction bioluminescence imaging, fluorescence positron emission tomography (PET), magnetic resonance (MRI). In analyses highlighted relationship between hypoxic conditions after pharmacological treatments cells. Through vivo studies, it possible assess establishment growth optical PET MRI. findings indicate that orthotopic murine can be reliably processes modulating using both preclinical non-invasive
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