Altered Middle Ear Microbiome in Children With Chronic Otitis Media With Effusion and Respiratory Illnesses.

摘要: Chronic otitis media with effusion (COME) is a common childhood disease characterized by an accumulation of fluid behind the eardrum. COME often requires surgical intervention and can also lead to significant hearing loss subsequent learning disabilities. Recent characterization middle ear (MEF) microbiome in pediatric patients has led improved understanding microbiota present during COME. However, it not currently known how MEF might vary due other conditions, particularly respiratory disorders. Here, we apply amplicon sequence variant (ASV) pipeline 16S rRNA high-throughput sequencing data from 50 children (ages 3-176 months) undergoing tube placement. We achieve more detailed taxonomic resolution than previously reported, including species genus level resolution. Additionally, provide first report functional roles demonstrate that despite high diversity, capacity remains uniform between patients. Furthermore, analyze differences without history lower airway (i.e., asthma or bronchiolitis). The was less diverse participants without, phylogenetic β-diversity (weighted UniFrac) significantly different based on status. Differential abundance those observed for genera Haemophilus, Moraxella, Staphylococcus, Alloiococcus, Turicella. These findings support previous suggestions link illnesses emphasize need future study tract microbiomes diseases such as bronchiolitis.