Integration of sequential analytical processes into sub-100 nm channels: volumetric sampling, chromatographic separation, and label-free molecule detection.

摘要: The progress of nanotechnology has developed nanofluidic devices utilizing nanochannels with a width and/or depth sub-100 nm (101 channels), and several experiments have been implemented in ultra-small spaces comparable to DNAs proteins. However, current 101 channels focus on single function or operation; integration multiple analytical operations into using circuits fluidic control yet be realized despite the advantage nanochannels. Herein, we report establishment label-free molecule detection method for demonstration sequential processes integrated devices. Our absorption-based called photothermal optical diffraction (POD) enables non-invasive first time, limit (LOD) 1.8 μM is achieved 70 wide deep nanochannels, which corresponds 7.5 molecules volume 7 aL. As sampling channels, aL-fL volumetric performed 90 cross-shaped pressure-driven from three directions. Finally, POD are combined nanochannel chromatography, separation analysis demonstrated. experimental results reported this paper will contribute advances potential provide novel platform chemical/biological analyses.