Chitosan-Based Vector/DNA Complexes for Gene Delivery: Biophysical Characteristics and Transfection Ability
作者： Patrick Erbacher , Shaomin Zou , Thierry Bettinger , Anne‐Marie Steffan , Jean‐Serge Remy
关键词:
摘要: Purpose. Chitosan, a natural cationic polysaccharide, is candidate non-viral vector for gene delivery. With the aim of developing this system, various biophysical characteristics chitosan-condensed DNA complexes were measured, and transfections performed. Methods. Transmission electronic microscopy (TEM) visualizations, sedimentation experiments, dynamic light scattering (DLS), zeta potential measurements realized. Transfections made by using luciferase reporter gene. Results. In defined conditions, plasmid formulated with chitosan produced homogenous populations which stable had diameter approximately 50−100 nm. Discrete particles nicely condensed donut, rod, or even pretzel shape. Chitosan/DNA efficiently transfected HeLa cells, independently presence 10% serum, did not require an added endosomolytic agent. addition, expression gradually increased over time, from 24 to 96 hours, whereas in same conditions efficacy polyethylenimine-mediated transfection dropped two orders magnitude. At was found be 10 times more efficient than PEI. However, chitosan-mediated depended on cell type. This dependency discussed here. Conclusions. Chitosan presents some favorable delivery, such as ability condense form small discrete conditions.
springer.com
sci-hub.se 参考文章(19)
Brambilla E, Brisson C, Steffan Am, Perron H, Oberling F, Furstenberger P, Labat-Moleur F, Behr Jp, Feugeas O, An electron microscopy study into the mechanism of gene transfer with lipopolyamines. Gene Therapy. ,vol. 3, pp. 1010- 1017 ,(1996)
Fred D. Ledley, Pharmaceutical approach to somatic gene therapy. Pharmaceutical Research. ,vol. 13, pp. 1595- 1614 ,(1996) , 10.1023/A:1016420102549
G.Y. Wu, C.H. Wu, Receptor-mediated in vitro gene transformation by a soluble DNA carrier system. Journal of Biological Chemistry. ,vol. 262, pp. 4429- 4432 ,(1987) , 10.1016/S0021-9258(18)61209-8
A. S. Chuck, M. F. Clarke, B. O. Palsson, Retroviral Infection Is Limited by Brownian Motion Human Gene Therapy. ,vol. 7, pp. 1527- 1534 ,(1996) , 10.1089/HUM.1996.7.13-1527
J Murata, Possibility of application of quaternary chitosan having pendant galactose residues as gene delivery tool Carbohydrate Polymers. ,vol. 29, pp. 69- 74 ,(1996) , 10.1016/0144-8617(95)00144-1
K. A. Mislick, J. D. Baldeschwieler, Evidence for the role of proteoglycans in cation-mediated gene transfer Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 12349- 12354 ,(1996) , 10.1073/PNAS.93.22.12349
J. P. Behr, B. Demeneix, J. P. Loeffler, J. Perez-Mutul, Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 6982- 6986 ,(1989) , 10.1073/PNAS.86.18.6982
Patrick Erbacher, Annie Claude Roche, Michel Monsigny, Patrick Midoux, Putative role of chloroquine in gene transfer into a human hepatoma cell line by DNA/lactosylated polylysine complexes. Experimental Cell Research. ,vol. 225, pp. 186- 194 ,(1996) , 10.1006/EXCR.1996.0169
O. Boussif, F. Lezoualc'h, M. A. Zanta, M. D. Mergny, D. Scherman, B. Demeneix, J. P. Behr, A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine Proceedings of the National Academy of Sciences of the United States of America. ,vol. 92, pp. 7297- 7301 ,(1995) , 10.1073/PNAS.92.16.7297
M. Cotten, F. Langle-Rouault, H. Kirlappos, E. Wagner, K. Mechtler, M. Zenke, H. Beug, M. L. Birnstiel, Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 4033- 4037 ,(1990) , 10.1073/PNAS.87.11.4033