Methods for extraction of microorganism DNA from glacier surface snow
作者： Yan Pei-Ying , Hou Shu-Gui , Chen Tuo , Zhang Shu-Hong , Sun Wei-Jun
DOI: 10.3724/SP.J.1226.2012.00484
关键词:
摘要: In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened (the Bosshard-Bano method Zhou method) optimized for most effective form filter membrane (cut vs. uncut), method, precipitation method. The then compared commercial Mo-Bio kit, results showed that kit was generally suitable microorganism from surface snow. Procedurally, it found a modified (i.e., cutting into pieces, using specific pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing only once by chloroform-isoamyl alcohol (24:1), conducting pure ethanol) also an less expensive
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