Soil washing improves the recovery of total community DNA from polluted and high organic content sediments

摘要: Treatment of soil with surfactants and chelating agents is used in bioremediation studies to desorb solubilize contaminants increase their bioavalability microorganisms. In the same way that pollutants are made more bioavailable microorganisms, procedure can be remove potential interfering materials from prior cell lysis extraction DNA indigenous The effect washing was evaluated by extracting sediments an intertidal freshwater wetland contaminated hydrocarbons highly marine Sydney Harbour, Nova Scotia, Canada. Sediment samples had total organic carbon (TOC) contents varied between 0.2% 13%. chemical technique also examined comparison ammonium acetate precipitation proteins humic acids a hexadecyltrimethylammonium bromide (CTAB) incubation phenol:chloroform extraction. this study, incorporation steps facilitated desorption sediment surfaces improved recovery amplifiable quality both sediments. CTAB contributed only slightly higher most sample Harbour concomitant decrease yield types. step polluted environments may important when high-quality required for subsequent analyses.