Immunogenicity and specificity of collagen. V. Demonstration of three different antigenic determinants on calf collagen.

摘要: Eighty rabbits were immunized with native or denatured acid-soluble calf skin collagen. Fifty-five antisera showed haemagglutination titres higher than 1:64. These sera investigated collagen, pepsin-treated collagen and rabbit used as coating antigens inhibitors. Three serologically distinct collagen-antibody fractions could be demonstrated: a general, non-species specific fraction reacting all three antigens, species active the two preparations, an antibody to pepsin-labile structures of only untreated The occur in either alone mixtures. present mixed determined by haemagglutination—inhibition experiments. Antibodies equally well inhibited peptides obtained after trypsin collagenase treatment. A ten-fold amount compared undergraded antigen was necessary obtain same inhibition effect. results previously reported peptide experiments performed other types antibodies.