Profiling cellular morphodynamics by spatiotemporal spectrum decomposition

作者: Xiao Ma , Onur Dagliyan , Klaus M. Hahn , Gaudenz Danuser

DOI: 10.1371/JOURNAL.PCBI.1006321

关键词:

摘要: Cellular morphology and associated morphodynamics are widely used for qualitative quantitative assessments of cell state. Here we implement a framework to profile cellular based on an adaptive decomposition local boundary motion into instantaneous frequency spectra defined by the Hilbert-Huang transform (HHT). Our approach revealed that spontaneously migrating cells with approximately homogeneous molecular makeup show remarkably consistent distributions, though they have markedly heterogeneous mobility. Distinctions in edge between these captured predominantly differences magnitude frequencies. We found acute photo-inhibition Vav2 guanine exchange factor, activator Rho family signaling proteins coordinating motility, produces significant shifts distribution, but does not affect magnitude. therefore concluded spectrum encodes wiring circuitry regulates movements, whereas captures activation level circuitry. also HHT as multi-scale spatiotemporal features statistical region merging identify subcellular regions distinct behavior. In line our conclusion different relate regimes, indeed exhibit Rac1 activities. This algorithm thus can serve accurate sensitive classifier pinpoint spatial temporal boundaries regimes.

参考文章(65)
Denis Tsygankov, Pei-Hsuan Chu, Hsin Chen, Timothy C. Elston, Klaus M. Hahn, User-friendly tools for quantifying the dynamics of cellular morphology and intracellular protein clusters Methods in Cell Biology. ,vol. 123, pp. 409- 427 ,(2014) , 10.1016/B978-0-12-420138-5.00022-7
Marco Vilela, Nadia Halidi, Sebastien Besson, Hunter Elliott, Klaus Hahn, Jessica Tytell, Gaudenz Danuser, Fluctuation analysis of activity biosensor images for the study of information flow in signaling pathways. Methods in Enzymology. ,vol. 519, pp. 253- 276 ,(2013) , 10.1016/B978-0-12-405539-1.00009-9
João M. Monteiro, Pedro B. Fernandes, Filipa Vaz, Ana R. Pereira, Andreia C. Tavares, Maria T. Ferreira, Pedro M. Pereira, Helena Veiga, Erkin Kuru, Michael S. VanNieuwenhze, Yves V. Brun, Sérgio R. Filipe, Mariana G. Pinho, Cell shape dynamics during the staphylococcal cell cycle. Nature Communications. ,vol. 6, pp. 8055- 8055 ,(2015) , 10.1038/NCOMMS9055
Mihaela Enculescu, Martin Falcke, Modeling Morphodynamic Phenotypes and Dynamic Regimes of Cell Motion Advances in Experimental Medicine and Biology. ,vol. 736, pp. 337- 358 ,(2012) , 10.1007/978-1-4419-7210-1_20
Michelle C. Mendoza, Marco Vilela, Jesus E. Juarez, John Blenis, Gaudenz Danuser, ERK reinforces actin polymerization to power persistent edge protrusion during motility. Science Signaling. ,vol. 8, ,(2015) , 10.1126/SCISIGNAL.AAA8859
Kwonmoo Lee, Hunter L. Elliott, Youbean Oak, Chih-Te Zee, Alex Groisman, Jessica D. Tytell, Gaudenz Danuser, Functional hierarchy of redundant actin assembly factors revealed by fine-grained registration of intrinsic image fluctuations Cell systems. ,vol. 1, pp. 37- 50 ,(2015) , 10.1016/J.CELS.2015.07.001
Matthias Krause, Alexis Gautreau, Steering cell migration: lamellipodium dynamics and the regulation of directional persistence Nature Reviews Molecular Cell Biology. ,vol. 15, pp. 577- 590 ,(2014) , 10.1038/NRM3861
R. Shlomovitz, N. S. Gov, Membrane waves driven by actin and Myosin. Physical Review Letters. ,vol. 98, pp. 168103- ,(2007) , 10.1103/PHYSREVLETT.98.168103
Leonard Bosgraaf, Peter J.M. van Haastert, Till Bretschneider, Analysis of cell movement by simultaneous quantification of local membrane displacement and fluorescent intensities using Quimp2. Cytoskeleton. ,vol. 66, pp. 156- 165 ,(2009) , 10.1002/CM.20338
Danying Shao, Wouter-Jan Rappel, Herbert Levine, Computational model for cell morphodynamics. Physical Review Letters. ,vol. 105, pp. 108104- 108104 ,(2010) , 10.1103/PHYSREVLETT.105.108104