A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells.

摘要: We recently reported that PIWI-interacting RNAs likes (piR-Ls) could regulate functions of the interacting phosphorylated proteins (p-Proteins). In addition, except for writers and erasers, functional efficacy p-Proteins on their readers still remains unknown. We, therefore, reasoned there was a type sncRNAs which p-Proteins. Here, we profiled with -Ser, -Thr -Tyr residues in 3 HBE 4 lung SCC cell lines, investigated effects mechanisms phosphorylated-residue-interacting sncRNAs. Our results demonstrated regulating thus referred them as Protein Functional Effector (pfeRNAs). pfeRNAs were distributed among 26 to 50 nucleotides, shared some core sequences showed distinctive expression patterns between cells. Core 417 (CS417), showing consistent upregulation all cells, bound directly p-Nucleolin (NCL), dependent key elements CGCG CS417 p-Ser619 NCL. The CS417/p-NCL interaction critical p-NCL basic activities normal cancer Thus, revealed novel controlling somatic