Impaired genome encapsidation restricts the in vitro propagation of human parvovirus B19.

摘要: The lack of a permissive cell culture system hampers the study human parvovirus B19 (B19V). UT7/Epo is one few established lines that can be infected with B19V but generates none or infectious progeny. Recently, hypoxic conditions use primary CD36+ erythroid progenitor cells (CD36+ EPCs) have been shown to improve infection. These novel approaches were evaluated in infection and transfection experiments. Hypoxic EPCs resulted significant acceleration infection/transfection modest increase yield capsid However, under all tested conditions, genome encapsidation was impaired seriously. Further analysis virus progeny revealed differently wild-type virus, VP1 unique region (VP1u) exposed partially unable become further externalized upon heat treatment. fivefold axes pore, which used for VP1u externalization encapsidation, might constricted by atypical conformation explaining packaging failure. Although hypoxia facilitate infection, large quantities cannot generated due failure arises as major limiting factor vitro propagation B19V.