Correlation between the abundance of yeast transfer RNAs and the occurrence of the respective codons in protein genes

摘要: Abstract There exists a similarity among the synonymous codon choice patterns of yeast nuclear genes that have been sequenced thus far although these encode different types protein molecules, and are significantly from those Escherichia coli genes. Based on constraints caused by availability E. transfer RNAs nature their recognition related to modified nucleotides at anticodon wobble position, characteristic commonly found for almost completely explained (Ikemura, 1981 a, b ). In present paper, tRNAs Saccharomyces cerevisiae were separated two-dimensional polyacrylamide gel electrophoresis relative abundance purified tRNA molecules was measured basis molecular numbers in cells. A strong correlation between each gene yeast, but less significant 2μ plasmid According criteria proposed ) order preference predicted based isoaccepting anticodons. Clear correlations predictions actual preferences codons revealed, indicating choices also constrained combination its recognition. Then difference use two organisms can be attributed factors.