The ITS region as a target for characterization of fungal communities using emerging sequencing technologies

摘要: The advent of new high-throughput DNA-sequencing technologies promises to redefine the way in which fungi and fungal communities – as well other groups organisms are studied their natural environment. With read lengths some few hundred base pairs, massively parallel sequencing (pyrosequencing) stands out among most apt for large-scale species identification environmental samples. Although pyrosequencing can generate hundreds thousands sequences at an exceptional speed, limited length reads may pose a problem process. This study explores whether discrepancy between traditional (Sanger) will have impact on perceived taxonomic affiliation underlying species. Based all 39 200 publicly available DNA representing nuclear ribosomal internal transcribed spacer (ITS) region, results show that two approaches give rise quite different views diversity Standardization subregion from ITS region should be sequenced, recognition composition depicted through methods need not directly comparable, appear crucial integration with current mycological praxis.