Early Embryonic Lethality Due to Targeted Inactivation of DNA Ligase III
作者： Nahum Puebla-Osorio , Devin B. Lacey , Frederick W. Alt , Chengming Zhu
DOI: 10.1128/MCB.26.10.3935-3941.2006
关键词:
摘要: DNA ligases catalyze the joining of strand breaks in phosphodiester backbone duplex and play essential roles replication, recombination, repair, maintenance genomic integrity. Three mammalian ligase genes have been identified, their corresponding distinct metabolism. III is proposed to be involved repairing single-strand breaks, but its precise role has not yet demonstrated directly. To determine cellular growth, embryonic development, we introduced targeted interruption (LIG3) gene into mouse. Mice homozygous for LIG3 disruption showed early lethality. We found that mutant developmental process stops at 8.5 days postcoitum (dpc), excessive cell death occurs 9.5 dpc. cells relatively normal XRCC1 levels elevated sister chromatid exchange. These findings indicate repair activities required development therefore cannot replaced by other ligases.
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