Targeting of interleukin-13 receptor on human renal cell carcinoma cells by a recombinant chimeric protein composed of interleukin-13 and a truncated form of Pseudomonas exotoxin A (PE38QQR).

摘要: We have previously shown that human renal cell carcinoma (RCC) cells express large numbers of interleukin-13 receptors (IL-13R), a newly described hemopoietic growth factor receptor. To target tumor IL-13R, we produced chimeric protein composed IL-13 and derivative Pseudomonas exotoxin A, termed PE38QQR. report here IL13-PE38QQR is highly cytotoxic to many RCC lines. IL-13R-negative lines or expressing low IL-13R ( < 300 sites/cell) include bone marrow-derived were not susceptible the effect IL 13-PE38QQR. The sensitivity correlated positively with density IL-13R. activity was competed by an excess in synthesis inhibition assay confirmed clonogenic assay. Even though IL-4 are homologues IL-4R been proposed share receptor subunit, did compete for cytotoxicity mediated IL13-toxin on RCC. competes [125I]-IL-13 binding sites cells, although at lower affinity than wild-type recombinant cytokine. Human T-cell, B-cell, monocytic unresponsive action IL13-PE38QQR. Thus, our results indicate it variety normal hematopoietic cells. should be further investigated preclinically treatment RCCs.