Mechanism of Action of Clostridium perfringens Enterotoxin

摘要: An enterotoxin produced by Clostridium perfringens during sporulation is a simple protein with molecular weight of about 35,000 and causative agent for the food poisoning organism (Todd, 1987). Production methods purification were studied intensively in 1960s 1970s (Duncan Strong, 1968; Stark Duncan, 1972; Sakaguchi et al., 1973; Granum Whitaker, 1980a; Sugimoto 1985a). These studies disclosed some unique characteristics protein, e.g. low solubility anomalous aggregation presence SDS (Enders 1976; 1980a,b). Recently, primary structure was clarified (Richardson Granum, 1985) gene encoding also cloned (Hanna 1989; Iwanejko Jongsten 1989). Thus, it expected that chemistry will clarify physicochemical background enterotoxin. On other hand, on biological action have made great progress last two decades. The purified shown to cause diarrhea experimental animals human volunteers (Hauschild 1971; Skjelkvale Uemura, 1977). It causes fluid accumulation ligated ileal loops rabbits mice when administered into lumen (Nillo, McDonel Besides gastrointestinal tract, reported kill rapidly intravenous or intraperitoneal injection (Genigeorgis Nillo, 1975). caused morphological alterations detachment mammalian cells culture (Keusch Donta, In this article, we overview at animal, organ, cellular levels.