Mapping and Functional Characterisation of a CTCF-Dependent Insulator Element at the 3′ Border of the Murine Scl Transcriptional Domain

摘要: The Scl gene encodes a transcription factor essential for haematopoietic development. is regulated by panel of cis-elements spread over 55 kb with the most distal 3′ element being located downstream neighbouring Map17, which co-regulated in cells. Scl/Map17 domain flanked upstream ubiquitously expressed Sil and cluster Cyp genes active liver, but mechanisms responsible delineating boundaries remain unclear. Here we report identification DNaseI hypersensitive site at end 45 start site. This boundary inactive chromatin, does not function as classical tissue-specific enhancer, binds CTCF both necessary sufficient insulator cells vitro. Moreover, transgenic reporter assay, expression promoter brain was increased incorporation 350 bp flanking fragments from +45 element. Our data suggests that region functions separates transcriptional domains, raise possibility this may be useful improving constructs.